摘要
目的探讨中药验方“龙泉复方制剂”的抑瘤作用及其作用机制。方法Lewis肺癌接种于DBA/2小鼠。S180肉瘤接种于BALB/C小鼠。将各味中药成分煮沸煎制,过滤后备用,每只鼠0.5ml/d,共10次。体外细胞毒性实验:MTT比色法。脾细胞细胞因子mRNA表达水平分析:RT-PCR。结果(1)本方对两种肿瘤都有明显的抑制作用,抑瘤作用与药量大小呈正比。同量的药对Lewis肺癌的作用更强一些。(2)用药后小鼠脾细胞对肿瘤细胞的杀伤率明显高于对照组;随着效靶比例的增加小鼠脾细胞对肿瘤细胞的杀伤率逐渐上升。(3)用药组脾细胞的IL-2和INF-γ的表达水平均明显增高。B7-H1的表达比对照组有所降低,但差异没有统计学意义。结论龙泉复方制剂是一种非常有前景的治疗肿瘤的复方中药,其作用机制可能是,对肿瘤细胞直接的毒性作用和通过促进免疫反应的间接抑瘤作用。
Objective To study the effect of Chinese medicine Longquan Fufang on tumor growth and its mechanism. Methods Lewis Lung cancer line was inoculated subcutaneously in DBA/2 mice. S180 cells was inoculated intramuscularly in BALB/c mice. The supernant of herbs （0. 5ml/day, each mouse） was given into mouse stomach for ten times. Cytotoxicity of CTL in vitro was measured with MTT colorimetry. The expression of mRNA was analyzed with RT-PCR. Results Longquan Fufang can inhibit significantly the growth of two cancer cell lines in mice. Its effect is dependant on dosage of herbs supernant used. The same dosage of herbs is more effective on Lewis lung cancer cells than on S180. After treatment with herbs, CTLs from mice spleen have stronger toxicity for tumor cells in vitro. The mRNA expression of IL-2 and IFN- was up-regulated by administration of herbs. The mRNA expression of B7-H1 did not changed significantly. Conclusion Longquan Fufang is a promising Chinese medicine for tumor therapy. Its mechanism could be direct toxicity for tumor cells and indirect inhibition by immune regulation.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2006年第4期255-257,共3页
Cancer Research on Prevention and Treatment
基金
湖北省科委科研基金资助项目(鄂科计〔2000〕4号)
