枸杞花药发育过程中脂滴和淀粉粒的分布特征

THE FEATURES OF DISTRIBUTION OF POLYSACCHARIDE AND LIPID IN THE DEVELOPING ANTHER OF LYCIUM BARBARUML.

宁夏枸杞(Lycium barbarurn L．)花药发育过程中,淀粉粒和脂滴两种营养物质的积累和分布具有一定的特点：在造孢细胞时期,药隔薄壁细胞,表皮和药室内壁细胞中开始积累淀粉粒,而造孢细胞、绒毡层细胞和中层细胞中则没有淀粉粒。在四分体时期,绒毡层细胞开始积累脂滴并且数量逐渐增加。到小孢子晚期,绒毡层细胞降解,内含脂滴流入药室中。在小孢子发育过程中既没有淀粉粒也没有脂滴积累,直到二胞花粉的大液泡消失后花粉粒中才开始积累脂滴,然后又开始出现淀粉粒。枸杞成熟花粉中的营养储存物是脂滴和淀粉粒。

Polysaccharide and lipid in the anthers of Lycium barbarurn L. at different stages were examined with cytochemical techniques. At the stage of sporogenous cell, many starches have been storied in parenchyma around vascular bundle; epidermis and endothecium cells but no starches in sporogenous cells, tapetal and middle layer cells. At the stage of tetrad, there were many starches still in epidermis and endothecium, however tapetal cells began to accumulate lipid drops, suggesting that tapetal cells transformed polysaccharides into lipid. Tapetum degenerated at the late stage of microspore and the lipid drops moved into locule. During microspore development neither starches nor lipid drops were accumulated in the cell. After the division of microspore, some lipids drops appeared in 2-cellular pollen, and then some starches also appeared in the pollen. Two days before anthesis, there were many lipid drops and starches located in nearly mature pollen grains, suggesting that pollen of Lycium barbarurn L. has a function of transforming lipid into polysaccharide. The temporal and spatial features of polysaccharide and lipid material accumulated and distributed in anther during its development reflect the transformation of physiological function of the cells consisting of anther. This research will help us to understand the mechanism of anther development.