细胞间黏附分子1基因K469E多态性与2型糖尿病肾病的相关性研究

Association of intercellular adhesion molecule-1 gene K469E polymorphism to type 2 diabetic nephropathy

目的探讨细胞间黏附分子1(ICAM-1)K469E基因多态性与2型糖尿病肾病 (DN)的关系。方法运用PCR-限制性多态性片断长度(RFLP)技术检测98名健康对照者和 210例2型糖尿病患者(其中DN患者110例,DM患者100例)的基因型,比较各组的基因型和等位基因频率。结果 (1)DN组KK基因型和K等位基因频率显著高于DM组和正常对照组: (2)与EE型组相比,KK型组DN的发生率明显上升;(3)Logistic回归分析显示ICAM-1 K469E 基因多态性是DN的危险因素。结论 ICAM-1 K469E多态性与2型糖尿病伴发肾病的发生有关,K等位基因可能是DN的易感基因。

Objective To evaluate the association between the K469E polymorphism in the intercellular adhesion molecule-1 （ICAM-1） gene and type 2 diabetic nephropathy （DN）. Methods According to their urine albmnin excretion rate （AER）,a total of 210 patients with type 2 diabetes were divided into type 2 diabetes with and without nephropathy groups. Another 98 controls with matched age and gender were selected from the same area. All patients and controls were genotyped by polymerase chain reaction （PCR） and digested with restriction endonuclease. Results The frequency of the KK genotype and K allele were significantly higher in the patients with diabetic nephropathy than those without nephropathy （genotype 36.4% vs. 22%, X^2=9.358 P=0.009; allele 60.9% vs. 46%,X^2=9.369 P=0.002）. Compared with genotype EE and KE class, DN morbidity in genotype KK class was significantly increased. Logistic regression analysis showed that the KK genotype was an independent risk for diabetic nephropathy in our population. Conclusions K469E polymorphism of ICAM-1 is associated with type 2 DN, and K allele may be susceptible to DN.