活体动物心脏植入材料细菌黏附的研究

Study on bacteria adhesion to intracardiac transplanted materials in vivo

目的研究体内心血管环境下细菌对心脏植入材料的早期黏附作用。方法53只兔分别通过外科手术植入涤纶片(DAC,n=16),戊二醛固定的自体心包片(GAP,n=18),冷冻保存的同种异体血管片(AG,n=19)于二尖瓣上,造成二尖瓣关闭不全。6h后经耳静脉注入5×106CFU金黄色葡萄球菌,48h后取出心脏,细菌培养并分析。结果10只兔早期死亡,弃用;DAC组中8/14只植入片细菌黏附阳性,细菌培养数目为(2.3×106±7.6×104)CFU/mg;GAP组中5/14只细菌黏附阳性,培养数目为(1.8×106±6.7×104)CFU/mg;AG组中6/15只细菌黏附阳性,培养数目为(4.9×106±1.0×104)CFU/mg。不同组间细菌培养数目差异无统计学意义(P>0.05)。结论心脏生物植入材料与人工材料相比在细菌早期黏附作用中并无明显的优越性。

Objective To study the early adhesive effect of bacteria on intracardiac transplanted materials in vivo. Methods Fifty-three rabbits were transplanted with different material intracardiac patches,eg, dacron （DAC ,n = 16） ,glutaraldehyde fixed autograft pericardium （GAP, n = 18） ,cryopreserved allograft （AG,n = 19）. The patches were transplanted on the mitral valve establishing mitral insufficiency. Staphylococcus auwus of 5 × 10^6 colony forming units （CFU） were injected 6 hours postoperatively into auricular vein. The rabbits were sacrificed 48 h after injection, then the patches were taken out from heart,cuUivated and analysed. Results Ten rabbits （2 DAC ,4 GAP and 4 AG） dead early and excluded from the study.Bacteria adhesive patches were found in 8/14 DAC,5/14 GAP and 6/15 AG groups. Differences of the CFU number in bacteria positive pathches were not significant among the different group （P 〉 0.05）. Conclusion The study demonstrates that there is no significant difference in the early effect of bacteria adhesion between intracardiac biological implants and artificial material prosthesis.