星点设计-效应面法优选人参叶酶法提取工艺

Optimization of Cellulase Hydrolysis Extraction Process of Folium Ginseng by Central Composite Design/Response Surface Methodology

目的:通过星点设计-效应面法优选人参叶酶法提取工艺。方法:用纤维素酶法从人参叶中提取人参总皂苷,并用星点设计-效应曲面分析方法进行多元线性回归和二项式拟合以分析酶反应参数(包括pH值,加水量,酶底比)对人参总皂苷得率的影响,从中选择最佳工艺,进行预测分析,得出预测值并与实际值比较。结果:二项式拟合复合相关系数较高,r为0.940,最佳提取工艺:pH为4.8,加水量为24倍,酶底比为40U/g,人参皂苷提取率实际值为6.29%,预测值为6.37%。结论:实际值和预测值吻合度高,星点设计-效应面法优选人参总皂苷酶法提取工艺是成功的。

Objective：To obtain the optimal cellulase hydrolysis extraction process of Folium ginseng by Central Composite Design/Response Surface Methodology. Methods： To extract ginsenosides from folium ginseng by adding eellulase digestion process. The parameters of enzyme hydrolysis action is divided into independent variables including pH value, water volume, the ratio of enzyme/the amount of herbal, and dependent variables-the extraction rates of ginsenosides, Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables. The response surface methodology was used to optimize the conditions of extraction. Prediction was carried out through comparing the observed and predicted values. Results： It was showed that the coefficient of correlation of second-order quadratic model was high. The correlation coefficient reached O. 940. The optimum conditions of extraction were obtained. The optimum condition was pH 4.8,24 times amount of water more than material medica, and enzyme/ substrate was 40U/g. The observed values and the predicated one of extraction ratio was 6.29 % and 6.37 %. Conclusion： The observed values and predicted one are close to each other, which prove that the optimization of cellulase digestion extraction process of ginsenosides from folium ginseng by Central Composite Design/Response Surface Methodology is reasonable and successful.