气血并治方及其配伍对高脂血症血瘀大鼠血管活性物质的影响

Effect of Qixuebingzhifang Extract on Vasoactive Substance and Activating Blood Circulation in Hyperlipidemia and Blood Stasis Rats

目的:观察气血并治方及方中活血药和理气药对大鼠脂质代谢、血管活性因子及活血化瘀的作用,比较全方及方中活血药和理气药的作用差异。方法:实验采用高脂饲料和应激方法造成大鼠高脂血症血瘀模型,灌胃给药2周后,检测大鼠血脂水平、血管活性物质含量和血液流变性指标的变化。结果:全方组、活血药组及理气药组对TC均有显著降低作用,作用强度依次为全方组、理气药、活血药,对LDL-C也有显著降低作用,和模型组比较有明显差异(P<0.05,P<0.01);理气药组和全方组对TG有显著降低作用,和模型组比较有明显差异(P<0.05,P<0.01);全方组能显著降低TC/HDL比值,和模型组比较有明显差异(P<0.01);全方组对内皮素(ET)含量有显著抑制作用,和模型组比较有明显差异(P<0.01),全方、活血药对血管紧张素II(AngII)有显著抑制作用。全方和活血药均有降低大鼠血液黏度、抑制血小板聚集性,降低红细胞聚集等作用。结论:气血并治方及方中活血药和理气药可作用于引起动脉粥样硬化的不同环节,二者配伍后的疗效在某些方面有一定增加;活血药组对血液流变性作用明显,全方组、理气药组次之,有效作用项目较少。

Objective： To observe the effects of prescription of treating both Qi and Blood, Qi Regulating Drugs and Blood Activating Drugs on lipid metabolism, vascular active factors of rats, compare the difference between the three agents. Methods： To establish the rat hyperlipemia and blood stasis model, rats were given stress with high lipid food. Plasm lipid, vascular endothelia function and hemorheology were observed in the three groups. Results： Compared with control group,all the three agents can significantly depress TC and LDL-c（ P 〈 0.05, P 〈 0.01 ） ; Qi-blood group and drugs for regulating Qi group can reduce TG, compared with control group there is markedly difference （ P 〈 0.05, P 〈 0.01 ）. Qi-blood group can significantly decrease TC/HDL ratio（ P 〈 0.01 ）. Compared with control group, Qi-blood group depressed endothelin （ET） level （ P 〈 0.01 ） ; Qi-blood and blood groups markedly reduce angiotensin Ⅱ （ Ang Ⅱ ） level （ P 〈 0.05, P 〈 0.01 ）. Qi-blood and blood agent can depress blood viscosity, restrain platelet aggregation ratio and erythocyte aggregation ratio. Conclusion： Qi-blood prescription and its Qi agent and blood agent can play various roles in targets relevant with a therosclerosis, the compatibility of Qi and blood agent can increase certain effects. Blood agent show more obvious effects in hemorheology than Qi-blood prescription and Qi agent.