郁金中姜黄素类化合物的毛细管区带电泳法测定

Separation and Determination of Curcumins in Curcma aromatica salisb by Capillary Zone Electrophoresis

本文在提取中药郁金中姜黄素类化合物的基础上,利用毛细管区带电泳法对郁金中所含黄素类化合物进行了分离研究,确定了最佳分离条件:9mmol/L单-6-O-苯基氨甲酰基-β-CD(mono-6-O-phenylcarbamoyl-β-CD),40mmol/L硼砂缓冲液,分离电压为15kV,毛细管柱温度为22℃,紫外检测波长为214nm,pH值为9.0。双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素在此条件下10 m in内能够达到基线分离,方法快速、简便、且消耗试剂少,不污染环境。

A capillary zone electrophoresis（CZE） method was developed for the determination and separation of curcumin, demethoxy curcumin and bis-demethoxy curcumin in the Chinese herbal extract in Curcuma aromatica salisb from Shandong, Jinan, Detection at 214 nm with a system containing sodium borate buffer and mono-6-O-phenlcarbamoyl-β-CD was found to be the most snitable approach for this analysis, The three curcumins could easily be determined within 10 minutes by this method. The effect of pH value, the concentration of the running buffer and mono-6-O-phenlcarbamoyl-β-CD on the migration time and resolution of the curcumins were also discussed.