摘要
目的:检测基质金属蛋白酶-2(MMP-2)在子宫内膜癌中的表达,分析其与临床病理特征间的关系,以探讨其在子宫内膜癌发生发展中的作用。方法:用免疫组化SP法检测45例子宫内膜癌中MMP-2的表达,另选12例增生子宫内膜和20例正常子宫内膜作为对照。结果:MMP-2蛋白在正常子宫内膜、增生子宫内膜中的表达无显著性差异(P>0.05)。子宫内膜癌组MMP-2表达高于对照组(P<0.05)。MMP-2在子宫内膜癌中、低分化组的表达高于高分化组(P<0.01)。在有肌层浸润标本中的表达高于无肌层浸润者(P<0.05)。结论:子宫内膜癌中MMP-2表达增强,与子宫内膜癌的发生、发展有关。
Objective: To detect the expression of matrix metalloproteinase - 2( MMP- 2)in endometrial carcinoma and study its relationship with clinicaopathological features so as to address the roles of MMP - 2 in the development of this malignant tumor. Methoils: A total of 45 endometrial carcinoma tissue blocks were obtained and stained using immunohistochemical streptavidin peroxidase technique with rnonoclonal antibody against MMP - 2, another 12 hyperplasia endometrium and 20 normal endometrium tissue blocks were used as control group.Results: There were no significant differences in the expression of MMP- 2 in normal endometrium and hyperplasia endometrium ( P 〉 0.05). The expression rates of MMP - 2 were signilicandy higher than that of control group ( P 〈 0.05) .The expression of MMP- 2 in group of middle and low differentiated carcinoma was significandy higher than that in group of highly differentiated carcinoma( P 〈 0.01 ), and the expression of MMP - 2 also significandy higher in the group with myometrium invasion than that in the group without myometrium invasion ( P 〈 0.05). Conclusion: MMP - 2 expression significantly increased in endometrial carcinoma, indicating that increased MMP- 2 may play a role in the development of endometrial carcinoma.
出处
《医学理论与实践》
2006年第4期391-393,共3页
The Journal of Medical Theory and Practice
关键词
子宫内膜癌
基质金属蛋白酶
免疫组织化学
Endometrial carcinoma, Matrix metalloproteinase, Immunohistochemistry