喉疾灵片中人工牛黄的检测

Identification and Determination of Artificial Cow-Bezoar in Houjiling Tablets

建立喉疾灵片中人工牛黄的质量控制方法。采用薄层色谱法鉴别人工牛黄特征成分贝斯素，方法简单、专属性强。高效液相色谱法测定胆红素的含量。采用Diamonsil^TM C18色谱柱，流动相为甲醇-氯仿-1％磷酸（81：13：6），流速为1．4ml·min^-1，检测波长为450nm。胆红素的线性范围为0．01589～0．1589μg，r=0．9993；平均回收率为96．20％，RSD为1．61％（n=9）。本方法可用于喉疾灵片的质量控制。

A quality control method was established for identification and determination of artificial Cow-Bezoar in Houjiling Tablets. The characteristic component Beisisu in artificial Cow-Bezoar was identified by simple and specific TLC method. Bilirubin was determined by HPLC. A Diamonsil ^TMC18 column was used with methanol--chloroform-1% phosphoric acid （81 ： 13 ： 6） as mobile phase. The flow rate was 1.4ml · min^-1 , and the detecting wavelength was 450nm. The linear range was 0.01589～0. 1589μg, r=0. 9993. The average recovery was 96.20%, RSD=1.61% （n=9）. The method could be used for quality control of Houjiling Tablets.