华东4个鹌鹑群体微卫星标记的遗传多样性检测(英文)

Detection of genetic diversity of four quail populations in East China based on three microsatellite markers

在DNA水平上检测了华东地区4个亲源关系不同的鹌鹑群体的3个微卫星座位遗传变异,每一位点均检测到4~5个等位基因,各位点的基因多态比例接近100%。为检测这一地区鹌鹑遗传多态性水平,估计了每个位点的基因杂合度和各群体的基因平均杂合度。结果表明:基因平均杂合度为(0.462 7±0.03)^(0.634 5±0.05),4个群体的平均值按由小到大排列分别为0.462 7、0.514 6、0.554 9和0.634 5,平均有效等位基因数为(1.868 8±0.12)^(2.798 1±0.43),平均多态信息含量为0.376 7~0.571 3,累积辨别力达到95.76%;聚类分析表明,华东地区鹌鹑群体间存在高水平的遗传变异,微卫星标记检测鹌鹑群体间的遗传多样性非常合适。

Three microsatellite loci were used with four quail （Coturnix spp. ） populations at their DNA level, in East China for the detection of genetic diversity of the no genetic relationship resources. Based on the microsatellites and data generated from the experiment, allele per locus detected ranged from four to five and proportion of polymorphism observed was in the neighbourhood of 100%. To detect the level of genetic diversity existing in quail populations in this region, we estimated gene diversity for each locus （h） and among loci （H）. The results demonstrated that （H） ranged from 0. 462 7± 0. 03 to 0. 634 5±0. 05 and the average gene diversity （H） observed in the populations were in the increasing order of 0. 462 7, 0. 514 6, 0. 554 9 and 0. 634 5, respectively. The range of mean of the effective allele number （Ne） was 1. 868 8±0. 12 to 2. 798 1±0. 43, mean polymorphism information content （PIC） was within 0. 376 7 to 0. 571 3 and cumulative power of discrimination across all populations gave 95.76%. The phylogenetic analyses of all data generated revealed that a high level of genetic diversity exists between the available quail populations in East China and that microsatellite markers are adequately suitable for examining genetic diversity of quail populations.