茶树对铝毒生理响应的研究

Physiological Response of Tea Plant to Aluminum Toxicity

以雁荡毛峰和知仁早茶两个品种为研究材料,研究茶树对不同浓度铝处理产生的生理响应,在加铝营养液培养50d后采集茶树第3～4片无损伤叶子测定丙二醛、过氧化物酶、过氧化氢酶的活力及游离辅氨酸含量。结果表明:低浓度的铝处理降低茶树的丙二醛含量,促进茶树过氧化物酶、过氧化氢酶活性和游离辅氨酸含量增加;高浓度的铝处理(100mg·L-1)降低了过氧化物酶的活性,提高了过氧化物酶活性和丙二醛含量;2个品种间对铝的反应存在着一定的差异,知仁早茶对铝的敏感性较雁荡毛峰大。

The physiological response of tea plant to aluminum （Al） was studied with two varieties of tea plant （Yandangmaofeng and Zhirenzaocha） by liquid culture. Four treatments of Al were designed, each one setting up two replicates： contrast group, liquid culturing with Hoagland nourishing oral liquid; Al2（SO4）3· 18H2O added to Hoagland nourishing liquid to make the final concentration of Al^3＋ 10 mg· L^-1, 50 mg·L^-1, 100 mg·L^-1. The content of malondialdehyde （MDA） and praline, the activities of peraxidase （POD） and catalase （CAT） were determined by gathering 3-4th leaf of tea tree after 50 days. The results showed that content of MDA in tea plant decreased, and content of praline, POD and CAT activities increased under the low concentrations of Al^3＋. However, POD activities decreased under the treatment of high Al （100 mg·L^-1）, and CAT activities and content of MDA increased. There were differences in physiological response between two varieties of tea and Zhirenzaocha was more sensitive to Al than Yandangmaofeng.