非小细胞肺癌组织中趋化因子受体CCR7、CXCR4表达及其临床意义

目的观察趋化因子受体CCR7、CXCR4表达与非小细胞肺癌(NSCLC)淋巴结转移的关系及其临床意义。方法采用免疫组织化学法检测64例～a期NSCLC患者癌组织石蜡包埋标本和9例癌旁正常肺组织标本中CXCR4、CCR7的表达。结果64例肺癌组织中39例呈CXCR4阳性,41例呈CCR7阳性;9例癌旁正常肺组织CXCR4、CCR7均阴性;CXCR4、CCR7表达均与淋巴结状态、临床分期有关,与年龄、性别、吸烟史、组织分级、局部浸润及病理类型无关;ROC分析显示,联合检测CXCR4、CCR7对诊断NSCLC淋巴结转移的准确性高。结论CXCR4、CCR7可能与NSCLC淋巴结转移有关,联合检测可预测NSCLC淋巴结转移情况及指导手术切除范围。

Objective： To investigate the relationship between chemokine receptor CXCR4 and CCR7 lymph node metastasis of NSCLC and their values in predicting lymph node metastasis. Methods, Immunohistochemistry was used to detect CXCR4 and CCR7 expression of paraffin＋embedded specimens from 64 NSCLC （Stage Ⅰ～Ⅱa）and 9 normal lung specimens taken from non-cancerous regions adjacent to lung cancer tissues. Results： In 64 lung cancer specimens, 39 cases were CXCR4-positive and 41 cases were CCR7-positive. But, no staining of CXCR4 and CCR7 was seen in 9 normal lung specimens. Expression of both CXCR4 and CCR7 was relevant to lymph node status and clinical stage. However, they were independent of age, gender, smoking, histological grade, regional invasion and pathological type. Receiver operating characteristic （ROC） analysis showed that the accuracy of the combined examination of CXCR4 and CCR7 expression in diagnosing lymph node metastasis was high（area under ROC curve[Az]= 0. 824）. Conclusion： The expression of CXCR4 and CCR7 may be related to lymph node metastasis of NSCLC. Moreover, the combined examination of CXCR4 and CCR7 may be useful in predicting lymph node metastasis of NSCLC and deciding the extent of surgical lymph node resection.