血管紧张素Ⅱ及其受体1在食管鳞癌血管生成中的作用

目的探讨血管紧张素(AngⅡ)及其受体1(AT1R)在食管鳞癌组织中的表达及其在血管生成中的作用。方法应用免疫组化SP法检测30例食管鳞癌患者癌组织及其食管断端正常鳞状上皮中Ang、AT1R和血管内皮生长因子(VEGF)蛋白的表达。结果Ang、AT1R和VEGF蛋白表达主要定位于鳞癌细胞;AngⅡ、AT1R在鳞癌组织中的表达均显著高于正常鳞状上皮(P均<0.01);癌组织中Ang的表达程度与VEGF的表达程度呈正相关(rs′=0.4249,P<0.05),AT1R的表达程度与VEGF无相关性(rs′=0.1298,P>0.05)。结论食管鳞癌癌组织中高水平表达AngⅡ及AT1R,二者可能通过诱导VEGF的产生促进肿瘤新生血管的形成。

Objective： To investigate the Angiotensin （Ang） -Ⅱ and Ang-Ⅱ type 1 receptor （AT1R） expression and their effects on angiogenesis in esophageal squamous cell carcinoma. Method： S-P immunohistochemical method was used to detect the expression of Ang-Ⅱ ,AT1R, and vascular endothelial growth factor （VEGF） proteins in 30 cases of esophageal squamous cell carcinoma specimens and their normal esophageal squamous epithelium. Results： Ang-Ⅱ ,AT1R, and VEGF proteins were mainly localized in cancer cells of esophageal squamous cell carcinoma; The expression of Ang-Ⅱ and AT1R were significantly higher in cancer tissues than that in normal esophageal squamous epithelium （both P〈0. 01）; The degree of Ang-Ⅱ expression had significantly positive correlation with that of VEGF expression in cancer tissues （ra′= 0. 4249,P 〈0. 05）. No significant correlation between the degree of ATIR expression and that of VEGF protein expression （ra′=0. 1298,P〉0. 05） Conclusion： The high level of Ang-Ⅱ and AT1R may promote the angiogenesis of esophageal squamous cell carcinoma through inducing the expression of VEGF.