摘要
采用水提醇沉法从芥菜中提取出水溶性粗多糖CP,经Sevag法脱蛋白和H2O2去色素,过DEAE-纤维素柱,经水洗涤和盐洗脱得到BPA,再经Sephadex G-100凝胶层析纯化得BPA 1.采用SephadexG-150凝胶过滤层析和醋酸纤维纸电泳鉴定,初步鉴定BPA 1为纯品.红外光谱检测证明BPA 1是多糖.
The water-soluble crude polysaccharide CP were obtained from the stalk of Brassica juncea by boiling-water extraction and ethanol precipitation. After the proteinic polysaccharides was removed by Savage way and the pigment in polysaccharides was removed by H2O2, CP was fractionated by DEAE-cellulose column chromatography, giving one polysaccharide fractions termed BPA. It was further purified with Sephadex-100 column chromatography, BPA1 was obtained. The Sephadex-150 gel filtration chromatography and cellulose acetate pellicle electrophoresis proved that BPA1 were purematerial.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第2期72-75,共4页
Journal of Fujian Normal University：Natural Science Edition
基金
华侨大学校基金资助项目(04X0008)
国务院侨务办公室基金资助项目(05Q0016)
关键词
芥菜
多糖
分离
鉴定
Brassica juncea
polysaccharides
isolation
identify
