部分省区啮齿动物中斑点热群立克次体的检测

Detection of Spotted Fever Group Rickettsia in Rodents in Several Regions,China

目的了解我国部分省区啮齿动物中斑点热群立克次体的自然感染情况,比较南北地区不同鼠种带菌率的差异。方法在黑龙江、内蒙古、新疆、浙江和贵州5省(自治区)共捕获鼠473只,用聚合酶链反应检测鼠脾脏中的斑点热群立克次体DNA序列片段。结果共检测鼠473只,69只阳性,阳性率为14.6%;在黑龙江、内蒙古、新疆、浙江和贵州省(自治区)都检测出阳性标本,阳性率分别为3.1%、21.6%、30.0%、10.8%和21.4%。结论我国黑龙江、内蒙古、新疆、浙江和贵州省(自治区)的鼠中都存在斑点热群立克次体的感染,南北地区鼠的带菌率差异无统计学意义(P>0.05)。

Objective To study the natural infection of spotted fever group rickettsia （SFGR） in rodents, and to compare the difference of the positive rates between different species of the southern and northern areas in China. Methods Rodents were collected in Heilongjiang, Inner Mongolia Autonomous Region, Xinjiang Autonomous Region, Zhejiang and Guizhou provinces. SFGR DNA sequence fragments in spleen of rodents was detected by PCR assay. Results The total number of 473 rodents were collected and detected, the positive rate was 14.6%. The positive samples came from Heilongjiang,Inner Mongolia Autonomous Region, Xinjiang Autonomous Region, Zhejiang and Guizhou provinces, with the infection rates of 3.1% ,21.6% ,30.0% ,10.8% ,21.4%, respectively. Conclusion There are SFGR infections in the rodents of Heilongjiang, Inner Mongolia Autonomous Region, Xinjiang Autonomous Region, Zhejiang and Guizhou province in China. The difference of the infections rates of both northern and southern areas are not significant（P 〉0.05）.