阿托伐他汀对动脉内膜损伤后血管平滑肌细胞离子泵活性和内膜增殖的影响

Influence of atorvastatin on ion pump activity of smooth muscle cells and intima proliferation after arterial intimal injury

目的:探讨阿托伐他汀对大鼠颈总动脉内膜损伤后血管平滑肌细胞离子泵活性和血管内膜增生的影响。方法:27只雄性SD大鼠随机分为假手术组(7只)、对照组和阿托伐他汀组(各10只)。后2组建立颈总动脉内膜损伤模型,阿托伐他汀组动物术前3d至术后28d每日接受阿托伐他汀(30mg·kg-1·d-1)治疗。术后28d处死大鼠,测定颈总动脉平滑肌细胞Na+K+ATP酶和Ca2+Mg2+ATP酶活性,应用苏木精伊红染色、计算机图像分析系统观察并测量动脉增生内膜厚度。结果:血管平滑肌细胞Na+K+ATP酶和Ca2+Mg2+ATP酶活性,阿托伐他汀组及假手术组均明显高于对照组(P<0.01)。对照组动脉最大增生内膜厚度明显高于阿托伐他汀组,P<0.01。结论:阿托伐他汀可能通过增高血管平滑肌细胞离子泵活性而抑制血管重塑的过程,从而在防治血管再狭窄中发挥作用。

Objective：To evaluate the influence of atorvastatin on ion pump activity of smooth muscle cells and intima proliferation in rats after arterial intimal injury. Method： Twenty-seven rats were divided randomly into sham group （ n =7）, control group （ n = 10） and atorvastatin group （ n = 10）. Animal models of carotid arterial intimal injury were established in the latter two groups, and those in atorvastatin group were administrated Atorvastatin 30 mg · kg^-1 · d^-1 from the third before the operation to the 28th day after the operation. On the 28th day, all of the rats were killed for the measurement of Na^＋-K^＋-ATPase and Ca^2＋-Mg%2＋-ATPase activities of smooth muscle cells from carotid artery. Intimal hyperplasia was observed with HE staining, and its maximum thickness were detected by a computer image analysis system. Result： Activities of Na^＋-K^＋-ATP ase and Ca^2＋- Mg^2＋-ATPase were significantly higher in sham and atorvastatin groups than those in control group, P 〈0. 01. The maximum thickness of intimal hyperplasia in control group was markedly higher than that in atorvastatin group, P 〈0.01. Conclusion： Atorvastatin might inhibit the process of vascular remodeling to prevent vascular restenosis by increasing ion pump activity of vascular smooth muscle cells.