紫外线减毒日本血吸虫尾蚴和童虫免疫小鼠的抵抗力研究

STUDIES ON RESISTANCE OF MICE VACCINATED WITH UV-ATTENUATED CERCARIAE AND SCHISTOSOMULA OF SCHISTOSOMA JAPONICUM

本文报告用２５条、５０条、１５０条和３００条紫外线减毒日本血吸虫尾蚴钻皮免疫或减毒童虫皮下注射免疫均能诱导小鼠产生有效的抗攻击感染抵抗力。以２５条免疫量为例，尾蚴钻皮及童虫皮下注射后的减成虫率分别为７２．１％和７０．４％，肝脏减卵率分别为６９．４％和５７．４％，小肠组织减卵率分别为７６．６％和８０．５％，免疫后的有效保护期为７．５个月。

Studies on mice vaccinated with UV-attenuated cercariae and schistosomula of Schistosoma japonicum were conducted,in which the optimal immunized dosage,route and effective protecting time were observed.The results showed that all groups could strongly be protected against challenges either using 25,50,150 and 300 cercariae percutanously or schistosomula injected subcutaneously.When the mice exposed to 25 vaccinating cercariae or schistosomula,they showed 72.1% and 70.4% reduction of the challenge worm burdens,69.4% and 57.4% reduction of eggs in liver,76.6% and 80.5% reduction of eggs in small intestines,respectively.The effective protecting time after vaccination was found to be 7.5 months.