细胞内游离Ca^(2+)的荧光指示剂研究进展

Development of Fluorescent Indicators of Intracellular Free Ca^(2+)

Ca2+在细胞的生命活动中起着重要作用,游离Ca2+浓度的变化与细胞的功能、信号传递及其损伤和凋亡都有着密切的联系,故胞内游离Ca2+浓度(简写为[Ca2+]i)的含量及其时空分布的精确测定极为重要.对目前用于测定细胞内游离Ca2+浓度的常见荧光指示剂进行了综述,分析了常用的研究方法并对其优、缺点进行了简单的比较和评述.

Ca^2＋ plays a very important role in regulating numerous physiological cellular phenomena and there is a close relation between the change in the concentration of free Ca^2＋ and the function of the cell, signal transmission and the cell injury and death. So it is very important to determine the concentration and the distribution of the intracellular free Ca^2＋. The common fluorescent indicators of intracellular free Ca^2＋ are summarized and the popular methods and their relevant merits and demerits were compared and analyzed.