人血浆中头孢氨苄浓度高效液相色谱法测定

Determination of cefalexin level in human plasma by reversed-phase high performance liquid chromatography

目的建立高效液相色谱法测定人血浆中头孢氨苄的浓度。方法采用噻克硝唑为内标，色谱柱为YMCODS-AC18（150mm×4．6mm），流动相为乙腈：甲醇：pH4．5的醋酸盐缓冲液（6：10：84），流速为1．0mL／min，检测波长为260nm。结果头孢氨苄血清浓度在0．101-32．100μg／mL范围内具有良好线性关系，最低定量限为0．101μg／mL，方法回收率为97．5％103．0％，日内、日间相对标准差均〈7％。结论高效液相色谱法简便、准确、灵敏，适用于头孢氨苄血药浓度监测和药代动力学研究。

Objective To develop a reversed-phase high performance liquid chromatography （RP-HPLC） method for the determination of cefalexin concentration in plasma. Methods The internal standard was secnidazole, the column was YMC ODS-A C18 （ 150 mm × 4.6 mm）, and the mobile phase consisted of acetonitrile, methanol alcohol and acetate buffer. The flow rate was 1.0 mL/min. The detection wavelength was 260 nrn. Results The calibration curves were linear when the cefalexin concentration in serum was in range of 0. 101 - 32.100 μg/mL, the limit of detection was 0.101 μg/mL, the method recovery rate was 97.5% -103.0%, the relative standard deviation of within-day and between-day were less than 7%. Conclusion The RP-HPLC method is simple, accurate and sensitive, it is suitable for monitoring the plasma concentration and clinical pharmacokinetics study of cefalxin in human.