转双价基因抗虫棉对斜纹夜蛾实验种群的影响

Effects of Zhongmiansuo 45 and Zhongmiansuo 41 on experimental population of Spodoptera litura

室内应用中棉所45、中棉所41两种转双价基因棉品种与对照品种中棉所23的顶端展开的第4张叶片,分别饲喂斜纹夜蛾Spodoptera litura幼虫,考察对幼虫、蛹、成虫的影响,编制并分析实验种群生命表,明确上述两种转双价基因棉对斜纹夜蛾的抗性作用。研究结果表明,两种转双价基因棉除中棉所41饲喂斜纹夜蛾2龄幼虫死亡率极显著高于中棉所23外,其他龄期幼虫死亡率、化蛹率及幼虫成蛾率均无显著差异;除中棉所41饲喂的斜纹夜蛾初孵幼虫历期显著长于中棉所23、5龄幼虫显著短于中棉所23,两种转双价基因棉饲喂的4龄幼虫历期显著短于中棉所23、蛹重显著轻于中棉所23外,其他龄期幼虫、蛹期、成虫产卵前期、产卵期、平均产卵量和雌成虫寿命均无显著差异。中棉所45、中棉所41和中棉所23饲喂斜纹夜蛾的净增长率(R0)分别为186.8721、236.7585、264.2246;世代平均周期(T)分别为45.6706、43.2262、46.8908;内禀增长率(rm)分别为0.1145、0.1265、0.1189;周限增长率(λ)分别为1.1213、1.1348、1.1263;种群加倍时间(DT)分别为6.0537、5.4794、5.8297;种群趋势指数(I)分别为237.70、315.24、319.28。综合分析中棉所45、中棉所41对斜纹夜蛾抗性不明显。

By feeding Spodoptera litura with the fourth leaf from top of two transgenic double-gene cottons （ Zhongmiansuo 45 and Zhongmiansuo 41 ） and Zhongmiansuo 23, effects of the transgenic cottons on larvae, pupae and adults of the pest were studied. The life table of an experimental population was established and analysised to evaluate the resistance. The results indicated that larval mortality of the transgenic cottons on the insect of the second instar feeding with Zhongmiansuo 41 was significant higher than on Zhongmiansuo 23, and the mortality of the rest of instar larvae, the period of larvae, the pupate rate of larvae and the rate of larva developed into moth of S. litura had no significant difference compared with control. The growth stage of hatched larvae and fifth instar larvae of S. litura feeding with Zhongmiansuo 41 were longer and shorter than that on Zhongmiansuo 23 respectively and the growth stage of fourth instar larvae and pupae weight fed on Zhongmiansuo 45 and Zhongmiansuo 41 were shorter and lower than that on Zhongmiansuo 23 respectively. While the growth stage of larvae, the period of pupae, mean preoviposition period, oviposition duration, mean fecundity and life-span of female adult had no significant differences. The net reproductive rates（ R0 ） of S. litura feeding with Zhongmiansuo 45 and Zhongmiansuo 41 and Zhongmiansuo 23 were 186. 8721,236. 7585,264. 2246 respectively; the mean generation times（T） were 45. 6706, 43. 2262, 46.8908 respectively; the intrinsic increase rates （rm ） were 0. 1145, 0. 1265, 0. 1189 respectively; the finite increase rates（λ） were 1. 1213, 1. 1348, 1. 1263 respectively; the dou- bling times （DT） were 6. 0537, 5. 4794, 5. 8297 respectively and index of population trend （Ⅰ） were 237.70, 315.24, 319.28 respectively. In general, resistances of Zhongmiansuo 45 and Zhongmiansuo 41 to S. litura were not significant.