HPLC法测定西替伪麻缓释片含量

Determination of cetirizine hydrochloride and pseudoephedrine hydrochloride sustained-release tablets by HPLC

目的 建立高效液相色谱法测定盐酸西替利嗪和盐酸伪麻黄碱含量的方法。方法 采用ODS柱（150mm×4．6mm），以甲醇-0．05mol·L^-1磷酸二氢钾（70：30）（每1000ml加入十二烷基硫酸钠0．3g）为流动相，使用紫外检测器，于214nm波长处测定。结果盐酸西替利嗪的线性范围在1．0～20．0nag·L^-1（r=0．9999），RSD=0．92％（n=9），盐酸伪麻黄碱的线性范围在24～480mg·L^-1（r=0．9999），RSD=0．92％（n=9），检测限分别为0．1μg和1．2μg。结论 本法可对盐酸西替利嗪和盐酸伪麻黄碱进行同时测定，具有快速、简便、灵敏、准确的特点。

Aim To determine cetirizine hydrochlofide and pseudoephedrine hydrochloride by HPLC in cetirizine hydrochloride and pseudoephedrine hydrochloride sustained-release tablets. Methods Shim-pack CLC-ODS（ 150 mm ×4.6 mm,5 μm） with methanol - 0.05 mol μ （70： 30） as mobile phase, flow rate was 1.0 ml · min^-1, and detection wavelength was 214 nm. Results The linear cirrelation was observed in the range of 1.0 · 20.0 mg μ as cetirizine hydrochloride, and 24 - 480 mg μ as pseudoephedrine hydrochloride. The average recovery were 98.88% and 99.51%. Conclusion The method is simple, rapid, accurate and reliable.