摘要
采用活化酯法用甲基聚乙二醇(mPEG)对高比活的人绒毛膜促性腺素(HCG)纯品进行化学修饰,利用放射免疫测定法研究了HCG结合mPEG后对HCG免疫反应性的影响,发现mPEG对HCG的修饰位点结合可影响抗体对抗原表位的识别结合,间接证明mPEG-HCG比天然HCG降低了免疫原性。
HCG was highly purified by immuno affinity chromatography and mFEG was ctiweated byactivating ester technique.THen HCG was chemcally modified by activated mPEG.The eacytivity mPEG-HCGs were detested by RIA.It was found that mPEG binding to some certansites of HCG affects HCG antibody's discriminating and binding to its epitone .It indicates that im-munologenicity of mPEG HCG is Iess than that of nature HCG
出处
《中国生化药物杂志》
CAS
CSCD
1996年第2期56-59,共4页
Chinese Journal of Biochemical Pharmaceutics
