左旋咪唑迟发性脑病免疫功能的研究

Study of immunologic function in patients with levamisole induced delay encephalopathy.

用间接免疫荧光法测定48例左旋咪唑迟发性脑病(LIDE)患者外周血淋巴细胞亚群,同时用 ICS-Ⅱ分析仪以速率散射比浊法检测患者血清和脑脊液(CSF)配对标本的部分体液免疫指标。结果表明:LIDE 组 CD_3、CD_4、CD_(25)、CD_4/CD_8比值均显著高于对照组,CD_8低于对照组(P<0.001)。血清和CSF IgG、IgA、IgM 及 IgG 鞘内合成率(IgG-Syn)亦均明显高于对照组(P<0.001)。CD_(25)、IgG-Syn 与病情严重程度相关密切(r=0.75,0.79;P<0.001),而 CD_4/CD_8比值则与发病—缓解时间有关(r=0.82;P<0.001)。肾上腺皮质类固醇治疗后,随病情改善患者外周血淋巴细胞亚群及 CSF 中部分体液免疫参数亦同步改善,并趋正常。

We determined the counts of lymphocyte subpopulation in peripheral blood by fluorescence microscopic examination and some humoral immunological parameters in paired cerebrospinal fluid(CSF)and serum samples by rate-nephelometric immuno-chem- istry system from 48 patients with levamisole induced delay encephalopathy(LIDE).The re- sults showed:In cases during exacerbation stage,the counts of CD_3,CD_4,CD_(25),CD_4:CD_8 ra- tio increased(P<0.001)and counts of CD_8 decreased remarkably.Serum and CSF IgG, IgA,IgM,intrathecal IgG synthesis(IgG-syn)was significant higher than those in controls (P<0.001).There was significant correlation between the counts of CD_(25),IgG-syn levle and the clinical condition of LIDE(r=0.75,0.79;P<0.001).It was also found that CD_4: CD_8 ratio correlated with the duration of LIDE(r=0.82,P<0.001).After treatment with corticosteroid,the disease showed recovery,the counts of lymphocyte subset and some hu- moral immunological parameters in CSF tended to normalize.