用SOS显色法和Ames试验检测脾组织浸液的遗传毒性和抑制突变作用

GENOTOXIC ACTIVITYDETICTEDANDDECRESSEDINSPLEENTISSUEEXTRACTSBYTHEAMESTESTANDASOSCOLORIMETRICTEST

采用大肠杆菌ＰＱ３７的ＳＯＳ显色法和ＴＡ１００、ＴＡ９８菌株的Ａｍｅｓ试验检测动物碑组织浸液的遗传毒性。在两个测试系统中均呈阴性反应；用ＳＯＳ显色法测定浸液对茶陡酮酸、丝裂霉素Ｃ所诱导的ＳＯＳ应答以及Ａｍｅｓ试验测定浸液对由叠氮钠、敌克松所诱发的回变作用，有一定的抑制作用（Ｐ＜０．０１），其强度与浸液浓度呈正相关。

The genotoxin and antimutation of spleen tissue extracts were detected by theAmes and SOS colorimetric test. Salmonells typhimurium TA98,TA100 were selected as theAmes tester strain. Escherichia coli k 12 PQ37 was used in the SOS chromotest.Both of themwithout S9 mix.Negative responses were showed in two systems. When spleen tissue ex- tracts were incubuted with NaN3,Dexson,MMC and Nalidixic respectively,the mutagenic po- tency whether in Ames or SOS test all had a significantly decrease.(P<0.01)。