新建人膀胱癌细胞系BIU-87的染色体分析

CHROMOSOME STUDY ON A NEWLY ESTABLISHED HUMAN BLADDER CANCER CELL LINE BIU-87

新建的人膀胱癌细胞系命名为BIU-87,本文研究其染色体及其G带的变化及临床意义。BIU-87细胞有明显的超4倍体特点,分析100个中期细胞得出染色体众数平均为101条。染色体形态结构与正常细胞相比有很大不同,可检出12个异常的标记染色体。其中最为明显的M_1和M_2标记染色体都与1号染色体有关。这将有助于临床辅助诊断膀胱癌。双小体的存在提示了细胞癌变和癌基因变化之间的关系。

A newly established cell line, derived from human bladder carcinoma, was designated as BIU-87.Its chromosome G-banding karyotype and clinical implication were studed. The chromosome number ofthe BIU-87 was characterized as hetertetraploid. By analysing 100 mitosis cells, the modal count was101 and the structure was quite diffrent from that of normal cell. A total of 12 marker chromosomeswas discovered. Notably. the M 1 and M 2 marker chromosomes are related to No. 1 chromosome. Weconclude that it could be of help to dignosis of bladder carcinoma. The presence of some doubleminute (DM) may imply the relationship between malignant cell and the oncogene.