摘要
通过厌氧堆肥试验,对厌氧堆肥产甲烷的基本特征进行了研究,结果表明:在厌氧堆肥开始阶段,气体中只有8%的甲烷,二氧化碳产率是甲烷产率的4倍左右;而随着反应的进行,二氧化碳产率呈下降趋势,甲烷产率逐渐升高,并于3个月时达到最高值45%;此后二氧化碳及甲烷产率都逐渐降低。对3个月时的垃圾堆肥渗出液取样,提取总DNA,对古细菌片段进行限制性片段长度多样性分析(RFLP),在60个随机选出的古细菌rDNA克隆子中,可以划分15个不同的谱型。对深入了解产甲烷厌氧微生物过程,加快垃圾稳定化具有重要意义。
Bench-scale composting experiment was made to study the features of methane produced in anaerobic digestion. Results indicated that with the processing of reaction, the output of CO2 decreased while methane increased to the maximum of 45% by the end of 3 months. RFLP analysis was also used to analyze Archaeal 16S rDNA genes that were selectively amplified from total genomic DNA extracted from microbial community in the leachate of compost for 3 months.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2006年第4期9-10,18,共3页
Environmental Science & Technology
基金
武汉市科技攻关资助项目(20026002095)
关键词
生活垃圾
厌氧
堆肥
甲烷
古细菌
RFLP分析
municipal solid waste
anaerobic digestion
composting
methane
Archaea
RFLP analysis
