昆明地区人群Calpain10基因SNP44变异与2型糖尿病遗传易感性的关联研究

A Study on the Correlation of Calpain 10 Gene SNP44 Polymorphism with Genetic Susceptibility to Type 2 Diabets in Kunming

目的探讨昆明地区人群Calpain10基因SNP44多态性与2型糖尿病遗传易感性的关系.方法采用1∶1病例对照研究方法,选取131例2型糖尿病患者和131例正常对照,以聚合酶链式反应-等位基因特异性扩增法(PCR-ASA),检测Calpain10基因SNP44T/C位点基因型,并进行关联分析.结果与正常对照组相比较,UCSNP44的C等位基因频率和TC基因型频率在2型糖尿病人群中显著升高,分别为18.7%及9.9%(χ2=8.232,P=0.004),37.4%和19.8%(χ2=9.882,P=0.002).结论Calpain10基因的SNP44变异与昆明地区人群2型糖尿病的遗传易感性相关.

Objective To explore the association of single nucleotide polymorphism -SNP44 of Calpain 10 gene with genetic susceptibility to type 2 diabetes in Kunming. Methods The polymorphism of site UCSNP44 （T/C） in Calpain 10 gene was detected by Polymerase chain reaction and Allele specific amplification （PCR- ASA） in 131 type 2 diabetic patients and 131 non- diabetic controls who were selected using casecontrol study in the Kunming urban communities. Results The frequency of ＂C＂ allele and ＂TC＂ gene of SNP44 in type 2 diabetic patients was significantly higher than that in the controls. Conclusion The study suggested that the polymorphism - SNP44 of Calpain10 gene may be associated with genetic susceptibility to type 2 diabetes in Kunming population.