电刺激小脑顶核对脑源性自主神经活性的保护作用

Electrical stimulation of cerebellum′s fastigial nucleus protects neurogenic autonomic function in rats after ischemic stroke

目的观察电刺激小脑顶核(FNS)对脑源性自主神经活性的保护作用。方法以大鼠右侧大脑中动脉阻塞为模型,用心率变异性(HRV)分析方法,观察脑梗死大鼠的心率变异性变化;将脑梗死大鼠随机分成非小脑顶核毁损组和梗死组,观察两组大鼠的心率变异性的频域和混沌参数值;用兴奋毒性物质鹅膏氨酸预先毁损两侧小脑顶核后,观察FNS对脑梗死大鼠的心率变异性的影响。结果大鼠脑梗死后,心率变异的频域和混沌参数值降低,与假手术组比较差异有统计学意义(P<0.05);当电刺激非小脑顶核毁损组大鼠小脑顶核时,发现心率变异的频域参数值、混沌参数值逐渐增加,与脑梗死组比较差异有统计学意义(P<0.05);预先毁损小脑顶核后,电刺激脑梗死大鼠小脑顶核,心率变异的参数值无明显变化,与非小脑顶核毁损组比较差异有统计学意义(P<0.05)。结论FNS可提高大鼠脑梗死后自主神经活性。这可能是其对抗缺血性脑损伤改善自主神经活性的脑保护作用之一。

Objective To observe the effects of electrical stimulation of the cerebellum＇s fastigial nucleus on neurogenic autonomic functional disturbances in rats after ischemic stroke. Methods Right middle cerebral artery occlusions （MCAOs） were performed on rats and their heart rate variability （HRV） was analysed. The MCAO rats were randomly divided into a group whose cerebellar fastigial nuclei （FNS） were stimulated and a control group. The power spectrum components and chaos of their heart rate variability were analysed. The MCAOs were performed after two cerebellar fastigial nuclei had been destroyed by pretreatment with ibotenic acid （IBO） , to investigate the effect of stimulating the cerebellum＇s fastigial nucleus on heart rate variability. Results The power spectral components and chaos in the heart rate variability of the MCAO rats were reduced, and there was a significant difference in the effectiveness rate compared with the sham groups （P 〈 0.05）. The cerebellum＇s fastigial nucleus in the MCAO rats, and the power spectral components and chaos in their HRV were higher when compared with the controls. There was a significant difference effectiveness rate （P 〈 0.05 ）. Stimulating the cerebellum＇s fastigial nucleus after excitotoxic lesions have been formed did not change the power spectral components and chaos in the HRV, but there was a significant difference in effectiveness rate compared with the no lesions group （ P 〈0.05）. Conclusion Stimulation of the fastigial nucleus of the cerebellum may benefit MCAO rats with autonomic functional disturbances by protecting brain tissue.