摘要
目的探讨超短波、旋磁治疗对大鼠局灶性脑缺血再灌注损伤的影响。方法应用线栓法制作大鼠局灶性脑缺血再灌注模型。将52只健康Wistar大鼠随机分为假手术组、对照组、超短波治疗组(超短波组)及旋磁治疗组(旋磁组)。脑缺血再灌注后18h给予治疗,24h取脑,观察各组大鼠脑组织含水量、脑梗死体积及Bcl-2、Bax蛋白表达,进行组间比较。结果脑缺血再灌注后18h,超短波治疗能减少脑含水量,减小梗死体积;超短波组与旋磁组Bcl-2的表达均提高,Bax蛋白表达降低,Bcl-2/Bax提高,超短波组与旋磁组间差异无统计学意义(P>0.05);在减少脑含水量和减小脑梗死体积方面,旋磁组与对照组比较,差异无统计学意义(P>0.05)。结论脑缺血再灌注后18h,应用超短波治疗对局灶性脑缺血再灌注损伤有显著疗效;本实验条件下旋磁治疗对改善脑水肿、减小脑梗死体积未见显著疗效,在改善凋亡方面有显著疗效。
Objective To investigate the effect and mechanism of ultrashortwave( USW ) diathermy and rotating magnetic( RM )field therapy on the brain following transient focal cerebral ischemia and reperfusion in rats. Methods Fifty-two Wistar rats were randomly divided into a sham operation group, an ischemia and a reperfusion control group, a USW treatment group and a RM treatment group. Focal cerebral ischemia and reperfusion were induced by intraluminal filament occlusion of the middle cerebral artery. Each brain was removed at 24 h after the reperfusion and water content, cerebral infarct volume and histological expression of Bcl-2 and Bax proteins were observed. Results When USW treatment was started at 18 h after reperfusion, decreased infarct volume and water content were observed, but RM treatment did not show this relationship. Both USW and RM were associated with in- creased expression of Bcl-2 protein and decreased the expression of Bax protein. Conclusions USW treatment is beneficial in focal cerebral ischemia. RM's effect is uncertain. Any effect is through reducing apoptosis.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2006年第4期225-228,共4页
Chinese Journal of Physical Medicine and Rehabilitation
基金
辽宁省社会发展基金资助项目(No.99225003)
关键词
脑缺血再灌注
超短波
旋磁
脑梗死
凋亡
Cerebral ischemia
Reperfusion
Ultrashortwave diathermy
Rotating magnetic field therapy
Cerebral infarct
Apoptosis