玉米芯水溶性多糖的分离纯化和抗凝血活性研究

The anti-coagulant effect of water soluble polysaccharides purified from corn-cob

从玉米芯中提取到水提水溶性粗多糖,通过乙醇分级、冻融、凝胶过滤层析,生物测定导向等手段,分离到一种能延长体外凝血时间,而且具有外源抗凝血功能的多糖CCⅢ.CCⅢ对活化部分凝血酶原时间(APTT)无显著影响,但可显著延长体外抗凝血时间(PT).将CCⅢ纯化,经糖组成分析、甲基化、高碘酸氧化、Smith降解和GC-MS分析,确定该多糖结构为:β-(1→4)Glc,(1→3)Xyl构成主链,Glc在6-O处有分枝.平均每10个糖残基有1个分枝,支链由β-(1→3)Xyl,(1→3)Glc构成.

Water-soluble crude polysaccharides have been extracted from corn-cob. The crude polysaccharide was further fractionated by ethanol and purified by Sepharose CL-4B gel filtration and the final products polysaccharides was named CC Ⅲ which could prolong the time of the coagulantion in vitro experiment. Smith degradation, methylation analysis and GC/MS were used to analyse the structure of CCX. The results revealed that CCX is a kind of lowly branched structure. The main chain is made up of （1→3）-linked-Xyl and β-（1→4）-linked-Glc, a few residues are substituted at 6-O. On the average, there is one branch among ten main chain residues. The side chain is composed of （1→3）-linkedGlc and （1→3）-linked-Xyl.