非洲菊工厂化育苗技术研究

The Technological Research on Tissue Culture Propagating by Factorizing Growing Seedlings of Gerbera Jamesonii

以非洲菊幼嫩花托为外植体,以MS为基本培养基,添加不同浓度6-BA、IBA、GA等进行愈伤组织诱导、不定芽分化、继代增殖、生根培养和过渡炼苗。试验表明:采用MS+6-BA 2.0～5.0 mg/L(单位下同)+IBA0.25～0.5培养基,愈伤诱导率在60%以上;采用MS+6-BA4.0+IBA0.25+GA0.5～2.0培养基对不定芽的生长及增殖效果好,增殖率可达13.5;采用1/2MS+NAA0.1或IBA0.1～0.5或IAA0.5～1.0培养基,20 d生根率达100%;在蛭石∶珍珠岩∶泥炭=1∶1∶1基质中驯苗,成活率可达95%以上。

Gerbera Jamesonii Bolus＇ tender receptacles were used as the explants and MS used as the basic culture medium, multiplication cultivation of making roots and culturing seedings of Gerbera Jamesonii Bolus were done in BA,IBA,GA of different density. The results show： In the culture medium of MS＋6-BA2,0mg/L-5.0mg/L＋IBA0.25mg/L-0.5mg/L,the callus rate is more than 60 %, InMS＋6-BA4.0＋IBA0.25＋GA0.5-2.0,the effect on multipcation and growth of sprouts is good, the multiplication rate reach 13.5 times;inl/2MS＋NAA0.1 orIBA 0.1-0.5 orIAA 0.5- 1 .0 , which makes the rooting rate reach 100 %during 20 days; if the sprouts are transplanted into the substrate in which the rate among vermiculite, perlite and peat is 1：1：1, the survival can reach over 95%.