摘要
以维生素Ｄ的体内生理活性形式－１．２５（ＯＨ）２Ｄ３作用于体外培养的小鼠成纤维细胞（Ｌ９２９细胞），然后用α－Ｐ３２标记的神经生长因子（ＮＧＦｃＤＮＡ）探针，检测Ｌ９２９细胞ＮＧＦｍＲＮＡ的变化。结果表明１．２５（ＯＨ）２Ｄ３作用３ｈ后，ＮＧＦｍＲＮＡ开始增加，并且随着１．２５（ＯＨ）２Ｄ３作用时间的增加及１．２５（ＯＨ）２Ｄ３作用浓度的增加，ＮＧＦｍＲＮＡ转录明显加强。提示１．２５（ＯＨ）２Ｄ３对神经生长因子基因的表达有促进作用。
The metabolically active form of vitamin D-1. 25 (OH)2D3 acted on the mouse fibroblasts L929 cells and then the changes of L929 cells nerve growth factor gene (NGF) mRNA in L929cells were detected with αP32labelled probe NGFcDNA. The results showed that 3 hours after 1.25 (OH)2D3 action NGF mRNA began to increase and increasingly transcribed with increasing time and concentration of 1.25(OH)2D3. The results show that 1.25 (OH)2D3 can promote the expression of NGF gene.
出处
《武警医学》
CAS
1996年第1期4-6,共3页
Medical Journal of the Chinese People's Armed Police Force
关键词
维生素D
神经生长因子
CDNA探针
NORTHERN杂交
Vitamin D Nerve growth factor gene cDNA probe Northern blot hybridization Seroid receptor superfamily
