WORT对血清饥饿及辐射诱导血清饥饿的SHG44增殖抑制作用的影响

Studies on Impact of Wortmannin on Inhibition Effect of Serum Starvation and Ionizing Radiation Exposing to SHG44 Human Glioma Cells

目的研究磷脂酰肌醇-3-激酶的相对特异性抑制剂WORT(Wortmannin,渥漫青霉素)对血清饥饿的SHG44细胞及辐射诱导血清饥饿的SHG44增殖抑制作用的影响。方法用甲基噻唑基四氮唑盐(Methyl thiazolyltetrazolium,MTT)比色法测定不同浓度的WORT处理的血清饥饿及血清饥饿后5 Gy照射的SHG44细胞的存活率。结果血清饥饿后,1.00×10-8mol/L^5.00×10-7mol/L的WORT使细胞存活率随WORT浓度的升高而降低(P<0.05),5.00×10-7mol/L^5.00×10-6mol/L的WORT使细胞存活率的降低出现平台期,1.50×10-5~3.00×10-5mol/L范围内细胞存活率再一次出现下降(P<0.05)。实验结果表明WORT可使血清饥饿后再接受5 Gy照射的细胞存活率进一步降低(P<0.05)。结论血清饥饿后,渥漫青霉素可抑制SHG44细胞的增殖,并提高辐射诱导血清饥饿细胞的增殖抑制作用,其机制可能与抑制PI3K通路以及PI3K家族其他成员相关。

Objective To study the inhibition effect of Wortmannin exposing to SHG44 cells after serum starvation and the impact of wortmannin on inhibition effect of ionization radiation exposing to the cells after serum starvation. Methods The survival rate of SHG44 cells after serum starvation and radiation was determined when they were treated with different doses of wortmannin with MTT assay. Results （1）The survival rate in the the group treated with wortmannin after serum starvation was significantly lower than that in the groups treated with serum starvation alone （ P 〈 0.01） ; （2） The survival rate in the group treated with 5 Gy radiation after serum starvation combined with wortmannin was significantly lower than that in the group treated with 5 Gy radiation after serum starvation alone（P 〈 0.01）. Conclusion wortmannin can suppress proliferation of SHG44 cells after serum starvation and enhance the inhibition effect of radiation exposing to SHG44 cells after serum starvation, which may be induced by suppressing PI3K pathway and other members of PI3K family.