TGF-β_1和CD105在胰腺癌中的表达及其临床意义

Study on the Expression and Clinical Significance of TGF-β_1 and CD105 in Pancreatic Carcinoma

目的探讨TGF-β1和CD105在胰腺癌生长、浸润、转移过程中的作用,为对患者进行预后判断及生物治疗提供理论依据。方法应用免疫组织化学S-P法检测TGF-β1、CD105在52例胰腺癌和10例非肿瘤性胰腺组织中的表达,分析它们与胰腺癌临床病理参数及患者生存率的关系。结果TGF-β1在非肿瘤性胰腺组织、胰腺癌中的阳性表达率分别为30%和80.77%,两者间的差异具有统计学意义(P<0.01)。TGF-β1的表达强度与胰腺癌临床分期及淋巴结有无转移呈正相关(P<0.01)。在52例胰腺癌中,CD105标记的微血管密度(Micro Vessel Den-sity,MVD)明显高于非肿瘤性胰腺组织(P<0.01)。胰腺癌分化程度越低,CD105标记的MVD值越高,并且TNM分期Ⅲ、Ⅳ期MVD值显著高于Ⅰ、Ⅱ期(P<0.01),淋巴结转移组的MVD值显著高于无淋巴结转移组(P<0.01)。此外,生存单因素分析TGF-β1、CD105与胰腺癌的预后有关(P<0.01)。结论TGF-β1和CD105在胰腺癌生长、浸润和转移过程中发挥了一定的作用。

Objective To study the effects of the expression of TGF-β1 and CD105 on growth, invasion and metastasis in pancreatic carcinoma, in order to provide some valuable evidence for prognosis and biotherapy. Method The expression level of TGF-β1, CD105 in the pancreatic carcinoma from 52 patients with pancreatic carcinoma and in the non-tumorous tissue in 10 cases was detected by S-P immunohistochemistry. Their relationship with clinic-pathological features and survival was also analyzed. Results The expression ratio of TGF-β1 in non-tumorous pancreas tissue and in pancreatic carcinoma was 30% and 80.77% respectively, having significant difference （P 〈 0, 01）. The TGF-β1 expression level positively correlated with clinical stages and metastasis of lymph nodes （P 〈 0.01 ）. The MVD marked by CD105 in 52 cases of pancreatic carcinoma was markedly higher than that in the non-tumorous pancreas tissue （P 〈 0.01）, The lower differentiation of pancreatic carcinoma, the higher MVD was. The MVD in stage Ⅲ ～ Ⅳ was evidently higher than that in stage Ⅰ ～ Ⅱ （P 〈 0.01）, and the MVD was also higher in those with metastasis of lymph node than those without metastasis （P〈 0.01）. The analysis of single factor revealed that the expression of TGF-β1, CD105 was significantly related with the prognosis of patients （P 〈 0.01）. Conclusion TGF-β1 and CD105 play an important role in growth, invasion and metastasis in pancreatic carcinoma.