豇豆再生体系的建立

Establishment of regeneration system for Vigna unguiculata

以丰产2号豆角下胚轴、上胚轴、茎段、真叶和顶芽作外植体进行了豇豆再生体系研究,结果表明,豇豆组织培养以愈伤组织诱导培养基和继代培养基为MS+6-BA 2.0 mg/L+NAA 2.0 mg/L,不定芽诱导培养基为MS+6-BA2.0 mg/L+NAA 0.2 mg/L,生根培养基为1/2MS+6-BA 0.2 mg/L+2,4-D 2.0 mg/L较适宜。

Regeneration system of Vigna unguiculata ＂Feng- chan 2＂ was established on different mediums in which contained different hormones, using different explants of this species such as hypocotyl, epicotyl, stem segment, leaf and terminal bud. The result showed that suitable medium of induction callus tissue and multiplication culture was MS ＋ 6 - BA 2. 0 mg/L ＋ NAA 2.0 mg/L, inducing medium was MS ＋ 6 - BA 2.0 mg/L ＋ NAA 0.2 mg/L,and roofing medium was 1/2MS ＋6-BA 0.2 mg/L＋2,4-D 2.0 mg/L.