摘要
以丰产2号豆角下胚轴、上胚轴、茎段、真叶和顶芽作外植体进行了豇豆再生体系研究,结果表明,豇豆组织培养以愈伤组织诱导培养基和继代培养基为MS+6-BA 2.0 mg/L+NAA 2.0 mg/L,不定芽诱导培养基为MS+6-BA2.0 mg/L+NAA 0.2 mg/L,生根培养基为1/2MS+6-BA 0.2 mg/L+2,4-D 2.0 mg/L较适宜。
Regeneration system of Vigna unguiculata "Feng- chan 2" was established on different mediums in which contained different hormones, using different explants of this species such as hypocotyl, epicotyl, stem segment, leaf and terminal bud. The result showed that suitable medium of induction callus tissue and multiplication culture was MS + 6 - BA 2. 0 mg/L + NAA 2.0 mg/L, inducing medium was MS + 6 - BA 2.0 mg/L + NAA 0.2 mg/L,and roofing medium was 1/2MS +6-BA 0.2 mg/L+2,4-D 2.0 mg/L.
出处
《广东农业科学》
CAS
CSCD
2006年第4期31-33,共3页
Guangdong Agricultural Sciences
基金
广东省自然科学基金项目(021272)
关键词
豇豆
外植体
愈伤组织
诱导
再生体系
Vigna unguiculata
explants
callus tissue
induction
regeneration system