木质纤维素稀酸水解液游离细胞乙醇发酵

ETHANOL PRODUCTION FROM DILUTE-ACID LIGNOCELLULOSE HYDROLYSATES BY FREE CELLS

为了对木质纤维素稀酸水解液进行游离细胞乙醇发酵,采用了混合菌种与不同发酵方式对稀酸水解液的乙醇发酵进行了研究。通过对1#菌和2#菌以及1#菌和3#菌两组混合菌种的驯化,得到了能耐受一定浓度的发酵抑制因子并产生较高乙醇产量的菌株。用1#菌和2#菌混合菌种以及1#菌和3#菌混合菌种进行批式发酵,72h内乙醇产率分别为0.49g/g和0.45g/g,达到了理论产率的96.1%和88.5%。对补料批式发酵进行的初步研究也取得了比较好的结果。

Mixed strain of free cells and different fermentative modes of batch and fed-batch fermentation were conducted to produce ethanol from dilute-acid lignocellulose hydmlysates. Before fermentation, the free cells were adapted, their ability to metabolize inhibitor was increased with increased batch time. Batch fermentation of dilute-acid lignocellulose hydmlysates gave an ethanol yield of 0.49g/g by 1^# and 2^# after 72h and 0.45g/g by 1^# and 3^# after 72h corresponding to 96.1% and 88.5 % theoretical ethanol yield. The astisfactory results were also achieved for the study of fed-batch fermentation.