氯乙醇对大鼠肝线粒体、微粒体膜脂流动性的影响

The influence of membrane fluidity in rat liver microsomesor mitochondria by chlorethanol

氯乙醇（ＣＥ）与肝线粒体或微粒体共同温浴，引起线粒体、微粒体的脂质过氧化（ＬＰＯ）表现为两二醛（ＭＤＡ）升高，同时，还引起荧光偏振度（Ｐ）降低（膜脂流动性升高），与对照组比较具有显著性差别，且存在着剂量－反应关系及时间－反应关系。在本实验条件下，发现巯基供剂二硫苏糖醇（ＤＴＴ）、过氧化物歧化酶（ＳＯＤ）对膜脂流动性均无保护作用，说明在该模型的实验条件下，荧光偏振度（Ｐ）的降低可能与脂质过氧化无关，可能由于ＣＥ既具有脂溶性又具有水溶性，能进入膜脂的中间层，破坏疏水环境所致。

The incubation of microsomes or mitochondria with chlorethanol(CE) resulted in the increase of malonyldialedehyde (MDA) and the decrease of fluorescence polarsation (p). Andthe changes showed significant time-and dose-dependence respectively. Dithiothreitol (DTT)or superoxide dismutase (SOD) could not protect the membrane fluidity although DTT couldantagonize lipid peroxidation.(LPO) caused by CE. The results suggested the increase of themembrane fluidity caused by CE was not associated with lipid peroxidation.