牛磺酸对肝微粒体膜的保护作用研究

Protective effects of taurine on rat hepato-microsomalmembrane in vitro induced by carbon tetrachloride

以四氯化碳（ＣＣｌ４，１０ｍｍｏｌ·Ｌ－１）与分离的大鼠肝微粒体共同温育（３７℃，１０，２０，３０，６０，１２０分钟），预先或同时加入牛磺酸（Ｔａｕｒｉｎｅ，Ｔａｕ），观察其对肝微粒体膜的保护作用。结果表明，ＣＣｌ４引起孵育体系中外源性加入的还原型谷胱甘肽（ＧＳＨ）、微粒体膜蛋白巯基（Ｐ－ＳＨ）含量降低，膜脂流动性（ＬＦＵ）降低，Ｔａｕ对此均有较明显的保护作用；在用Ｔａｕ预孵育的实验体系中，Ｔａｕ对ＣＣｌ４引起的ＬＦＵ降低的保护作用更加显著。在本实验条件下，未观察到脂质过氧化产物丙二醛（ＭＤＡ）含量的显著变化。这些结果提示Ｔａｕ对ＣＣｌ４引起的微粒体膜Ｐ－ＳＨ和ＬＦＵ改变有一定的保护作用。

The protective effects of taurine on rat hepato--microsomal membrane in vitro induced bycarbon tetrachloride (CCl4) at 37℃ at 10th, 20th, 30th, 60th and 120th minute were observed. The present results showed that CCl4 reduced the contents of glutathione (GSH) andprotein--thiol (P--SH). 10 mmol. L-1 taurine could inhibit the action of CCl4 on both GSH andP--SH,respectively. The membranous lipid fluidity (LFU) was declined by CCl4. When bothCCI. and taurine were added simutaneously,the protective role of taurine on LFU was shownat 10th minute but not at 30th minute. After preincubation with microsomes and 0. 5,2. 5 and10 mmol. L-1 taurine respectively,the changes of LFU induced by CCl4 were prevented bothat 10th and 30th minute. These results suggested that taurine was a membranous protectiveagent whose biological roles might include detoxication and antioxidation.