疏肝和胃丸体内外对幽门螺杆菌的抑制作用

Inhibitive Effect of Shuganhewei Pill on Helicobacter Pylori in Vivo or Vitro

目的:探讨疏肝和胃丸治疗幽门螺杆菌感染的急慢性胃炎、胃及十二指肠溃疡的作用机制。方法:体内抗菌试验采用幽门螺杆菌感染小鼠造模,体外抗菌试验采用血琼脂平板扩散法进行药物抗幽门螺杆菌实验观察,用细菌涂片、尿素酶试验(RUT)检验经疏肝和胃丸治疗后小鼠胃幽门部幽门螺杆菌的清除状况,在血琼脂平板中抗幽门螺杆菌实验测定疏肝和胃丸对幽门螺杆菌的最低抑菌浓度(M IC)。结果:疏肝和胃丸高剂量组能显著降低幽门螺杆菌感染小鼠胃幽门部幽门螺杆菌的阳性率,其作用与对照组阿莫西林相当。在血琼脂平板中疏肝和胃丸抗幽门螺杆菌测得M IC为0.0156g/m l生药,其抑菌作用随着药物浓度的增高而增强。结论:疏肝和胃丸能在体内外抑制或杀灭幽门螺杆菌。

Objective： To investigate the effective mechanism of Shuganhewei Pill on treatment of acute, chronic gastritis, gastric and duodenal ulcer caused by helicobacter pylori infection. Method： to adopt the mouse model infected by helicobacter pylori in vivo anti - helicobacter pylori experiment, to adopt blood agar plate diffusion process to carry on experimental observation of anti - helicobacter pylori in vitro anti - helicobacter pylori experiment, to use the piece with the bacterium and urease test to test the clear condition of helicobacter pylori in mouse pylorus after being treated by Shuganhewei Pill, at last, to detect the minimum inhibitory concentration （ MIC ） in above stated plate. Results ： High dosage group of Shuganhewei Pill could remarkable decrease positive rate of infecting helicobacter pylori mouse, the effection was equal to amoxicillin. The minimum inhibitory concentration in above stated plate was 0. 0156g/ml. and the anti -bacteria function was increasing with the augment of drug concentration. Conclusion： Shuganhewei Pill can inhibit or kill helicobacter pylori in vivo or vitro.