摘要
用圆二色光谱、荧光光谱及紫外差光谱研究镁钙离子诱导的FⅨ、PC的构象变化并通过蛋白负染电镜及荧光偏振实验观察了各构象与FⅨ、PC特异单抗的连接状况。FⅨ、PC在镁钙离子诱导下,其3种光谱均发生显著变化,并且与缓冲液中的金属离子浓度有关。FⅨ、PC的特异单抗仅与FⅨ、PC的某种特定构象发生连接反应。
The changes in conformations of Factor Ⅸ and protein C induced by metalions Mg(2+) and Ca(2+) have been studied with CD spectra,fluorescence spectra and UV difference spectra,and the experimental observation on the connexion of all conformations with factor Ⅸ-and-protein C-specific monoclonal antibodies has been taken using protein negative stained electron microscope and fluorescence polarization,indicating that the significant changes in conformations of factor Ⅸ and protein C occured when being induced by the same ions,depending upon the concentration of the same ions in buffer and that the connexion of factor Ⅸ-and-protein C-specific monoclonal antibodies could take place only with some particular conformation of factor Ⅸ and protein C.
出处
《中国输血杂志》
CAS
CSCD
1996年第1期2-5,共4页
Chinese Journal of Blood Transfusion
基金
国家自然科学基金