增殖细胞核抗原在恶性淋巴瘤中的表达及其意义

Expression and Significance of Anti-PCNA in Malignant Lymphomas

应用增殖细胞核抗原(PCNA)单克隆抗体对45例NHL、20例RH、7例HD石蜡包埋组织切片进行免疫组化(S—P法)染色,用半定量方法计阳性细胞数,并观察阳性细胞分布规律。结果显示:NHL中阳性细胞数与恶性程度高低有关。不同级别的NHL与RH组之间阳性细胞均值有显著性差异。认为PCNA单抗标记能较好地反映肿瘤增殖状态,对NHL分级及与RH的鉴别有一定价值,也是各型R—S细胞有效的标记物。

The immunohistochemical detection of McAb PCNA was used to study the prolif-erative activity of 45 non-Hodgkin' s lymphomas (NHL), 20 reactive hyperplasias (RH) and 7 Hodgkin' s diseases(HD) in formalin-fixed samples. The result showed that mean number of PCNA positive cells was significantly different between the NHL and RH and among different grades of NHL (P<0.01). It suggested that PCNA immunoreactivity can be used as an index of cell proliferation and might be helpful in differentiating the NHL from the RH. But also a good indicator for R-S cells.