小红参愈伤组织的诱导及分化

Callus Induction and Bud Formation ofRubia yunnanensis(Franch.) Diels

通过不同外植体、基本培养基、暗培养、激素对小红参愈伤组织诱导和分化的试验研究，结果表明诱导小红参愈伤组织产生的最佳条件为：以真叶和顶芽（或侧芽）为外植体，在MS＋BA0．2mg／L＋NAA3．0mg／L培养基上，进行暗培养，愈伤诱导率达95％。芽形成的愈伤组织组织在MS＋BA5．0＋NAA1．5～2．0中均能分化出幼苗，分化率达100％，但叶形成的愈伤组织未能分化出苗。

The effect of different plantlet , basic medium, dark culture , hormone combination and densities on the formation and differentiation of callus of Eucalyptus smithii L was studied. It was found that callus form well in the dark using true leaf and bud as plantlet, MS＋BA 0. 2 mg/L＋NAA 3. 0 mg/L as basic medium with the forming rate of callus up to 95%. The callus from the bud can differentiate to form bud using MS＋ BA 0. 5＋NAA 0. 5 as medium with the rate up to 100%. But the callus from the leaf can not differentiate to form adventitious bud.