精氨酸脱亚胺酶发酵条件研究

Study on Fermentation Process of Arginine Deiminase Producing Strain

对一种新型肿瘤增殖抑制酶类一精氨酸脱亚胺酶的发酵生产工艺进行了研究。运用单因子扫描方法，考察了不同碳源、氮源等影响因子对粪肠球菌（Enterococcus faecalis）NJ402产精氨酸脱亚胺酶的影响。优选出其较佳培养基组分为（g／L）；蔗糖15，蛋白胨5，酵母膏5，牛肉膏2．5，NaCl3，KH2PO42，MgSO40．01，MnSO40．0025，L-精氨酸15；接种量以4％～5％为宜，最佳培养温度是37℃，最适起始pH为7．5。100L发酵罐实验表明，发酵到10h时，菌量与比酶活量均为最大，分别为40mg／mL和2．57U／mL。

Process of fermentation of arginine deiminase, which was a new tumor-growth inhibitor, was studied. Effects of various carbon sources, nitrogen sources and other factors on arginine deiminase production in Enterococcus faecalis NJ402 were investigated in single factor test. The optimal medium was selected as follows （g/L）： sucrose 15, peptone 5, extract of yeast 5, extract of beef 2.5, NaCl 3, KH2PO4 2, MgSO4 0.01, MnSO4 0.002 5, L-arginine 15, pH 7.5. with 4% inoculum in 100L fermentor. E. faecalis could reach high yield of arginine deiminase after 10 h at 37℃, the specific activity could reach 2.57 U/mL with yield of 40 mg/mL biomass.