Y染色体非重组区HindⅢ酶切位点多态性与老年男性原发性高血压的关系

Polymorphic HindⅢ restricted site of the Y chromosome and essential hypertension in gerontal males

目的探讨Y染色体非重组区HindⅢ酶切位点多态性与老年男性原发性高血压的关系。方法入选60岁以上男性254例,其中正常人群(对照组)85例,原发性高血压患者(高血压组)169例。常规方法提取白细胞DNA。采用多聚酶链反应结合限制性内切酶(HindⅢ)方法检测Y染色体HindⅢ酶切位点多态性。结果对照组Y染色体HindⅢ酶切位点多态性与原发性高血压组基因型存在显著性差异(P=0.02),高血压病患者HindⅢ(-)基因型增多。HindⅢ(+)基因型较HindⅢ(-)基因型收缩压(145.1±25.7)mm Hg(1 mm Hg=0.133 kPa)vs(154.1±24.8)mm Hg,P=0.006,平均动脉压(105.9±15.4)mm Hgvs(110.7±14.9)mm Hg,P=0.019,脉压(58.8±17.6)mm Hgvs(65.1±18.7)mm Hg,P=0.007均明显降低。结论Y染色体HindⅢ酶切位点多态性与老年男性原发性高血压有关,其可能是老年男性原发性高血压的一个遗传标志。

Objectives To examine whether there is an association between BP and a polymorphic Hind Ⅲ biallelic marker in the nonrecombining region of the Y chromosome in Chinese gerontal males with essential hypertonsion.Methods Eighty-five healthy people above 60 years old were enrolled into this study as control group and 169 patients with gerontal essential hypertension were enrolled as case group. DNA was extracted from white blood cells. Segments of polymorphic Hind Ⅲ restricted site of the Y chromosome were amplified from DNA by polymerase chain reaction（PCR）. PCR products were restricted with 10 U of Hind Ⅲ for overnight at 37 ℃. The digested products were subjected to electrophoresis in 3 % agarose gels and stained with ethidium bromide. Results 1. The Hind Ⅲ （ - ） genotype was found in 29.4 % of the men in control group and 45.0% of the men with essential hypertension. The frequency of Hindm （ - ） genotype in men with essential hypertension was significantly higher than that in the control（ P = 0.02）. 2. The Hind Ⅲ （ ± ） genotype had lower SBP[ （ 145.1 ± 25.7） mm Hg vs （ 154.1 ± 24.8） mm Hg, P = 0. 006], pulse pressure[ （58.8 ± 17.6） mm Hg vs （65.1 ± 18.7） mm Hg, P = 0.007 ] and mean arterial pressure [ （ 105.9 ± 15.4） mm Hg vs （110.7 ± 14.9） mm Hg, P = 0.019] than the Hindm （ - ） genotype. Conclusion Polymorphic Hindm restricted site of the Y chromosome is possibly associated with gerontal male essential hypertension in China.