高效液相色谱法测定草莓中维生素C含量

Determination of vitamin C in strawberry by high performance liquid chromatography

目的：建立一种高效液相色谱测定草莓中维生素C含量的新方法．方法：采用HYPERSIL—C18色谱柱，0．1％低浓度的草酸作流动相，流速为1．0mL／min，检测波长为254nm．结果：维生素C在浓度为20—100mg／L范围内有良好的线性关系，线性回归方程为Y=9361．6x-11192，相关系数为O．9993，方法回收率为97．4—102．1％，相对标准偏差小于3％．结论：本测定方法简便，快速，准确，稳定；适合于测定其它水果和蔬菜中维生素C的含量．

[ Objective] A new method of detecting the conent of vitamin C in the strawberry by using HPLC is established. [ Methods] A HYPERSIL- C18 column is used and the flow phase is low concentration 0. 1% oxalic acid,the flow rate is 1.0 mL/min and the detection waveleng is 254 nm in the HPLC system. [ Results ] The standard working curve of vitamin C has a good linear relation in the concentration range from 20 - 100 mg/L,the linear equation is y = 9 361.6 x - 11 192 and the related coefficient is 0. 999 3. The recoveries are 97.4 - 102. 1% and the relatively standard deviation is below 3%. [ Conclusions] This method is simple and fast and exact and steady and suitable to detect the conent of vitamin C in other fruit and vegetable.