摘要
目的建立心血宁滴丸中金丝桃苷的含量测定方法。方法采用高效液相色谱法，以十八烷基硅烷键合硅胶为固定相，甲醇-乙腈-四氢呋喃-0．5％冰醋酸（1：1：30：70）为流动相，检测波长为363nm，测定金丝桃苷的含量。结果金丝桃苷进样量在79．6-318．4ng有良好的线性关系，回收率为98．8％±1．86％。结论本法测定心血宁滴丸中金丝桃苷的含量，结果准确可行。
OBJECTIVE To establish the assaying method for the determination of hyperoside in Xinxuening dropping pills. METHODS External standard method was conducted with the column of C18 （250 mm× 4. 6 mm, 5 mm）, mobile phase of methanol-acetonitrile-tetrahydrofuran-0. 5% acetic acid（1 ： 1 ： 30 ： 70）, flow rate of 1.0 mL ·min^-1, and hyperosidewas determined at the wavelength of 363 nm. RESULTS In the range of 79. 6±318. 4 ng , the distribution of hyperoside was linear （r= 0. 999 8）, with an average recovery of 98. 8 % ± 1.86 % （RSD= 2. 81%）. CONCLUSIONS The assaying method of RP-HPLC is convenient, accurate, and reliable with good precision, and can be used in the quality control of Xinxuening dropping pills.
出处
《中南药学》
CAS
2006年第2期106-108,共3页
Central South Pharmacy
基金
广东省中医药管理局课题(课题编号1040087)
