反相高效液相色谱法测定丹参滴注液中丹参素和原儿茶醛

Synchronous quantitative analysis of danshensu and protocatechuic aldehyde in danshen dripping solution by RP-HPLC

目的建立同时测定丹参滴注液中丹参素和原儿茶醛含量的方法。方法利用RP-HPLC法，采用Diamonsil^TM C18色谱柱（250mm×4．6mm，5μm），以甲醇-乙腈-冰醋酸-水（10：5：0．25：85）为流动相，流速为1．0mL·min^-1，检测波长为281nm。结果用RP-HPLC法测定2个活性成分丹参素和原儿茶醛分别在20．6～82．4μg·mL^-1和7．8～56．0μg·mL^-1与峰面积呈良好线性关系，相关系数分别为0．9990和0．9996。回收率丹参素为99．46％（RSD=1．0％，n=3）、原儿茶醛为99．96％（RSD=0．97％，n=3）。结论本方法简便、快速，两峰分离良好，可用于丹参滴注液的质量控制。

OBJECTIVE To determine the contents and determination method of protocatechuic aldehyde （PCAD） and danshensu in danshen dripping solution. METItODS RP-HPLC method was used. The column was Diamonsil^TM C18 （250 mm×4.6 mm, 5μm）. The mobile phase was methanol-acetonitrile-acetic acid glacial-water （10 ： 5 ： 0. 25 ： 85） ; the flow rate was 1.0 mL · min^-1 ; the detection wavelength was 281 nm. RESULTS The calibration curves of danshensu and PCAD were linear in the range of 20.6-82.4μg · mL^-1 and 7.8-56. 0μg · mL^-1 with the correlation coefficient beyond 0. 999 0 and 0. 999 6 respectively. The mean recoveries of the assay were 99.46% （RSD= 1.0%, n=3） for danshensu and 99.96 % （RSD=0.97%, n=3） for PCAD respectively. CONCLUSIONS This method is simple, quick with good separation, and it is useful in the quality control of danshen dripping solution.