HBV在慢性乙型肝炎患者肝细胞染色体上整合情况的临床分析

Detection of HBV virus integration in hepatacellular chromosome of the patients with chronic hepatitis B using fluorescence in-situ hybridization

采用荧光原位杂交（FISH）技术检测慢性乙型肝炎患者肝细胞染色体，明确是否存在HBV的整合及整合情况。选择我院慢性乙型肝炎患者共16例，制备其肝细胞染色体标本，以地高辛标记的HBV探针进行原位杂交。16例患者中，有5例患者的肝细胞分裂期染色体上可观察到绿色荧光信号，占31．25％，HBV呈低频率整合（1．00％-1．50％），整合位点呈随机分布。通过研究发现，应用FISH技术可检测肝细胞染色体上整合型HBV。慢性乙型肝炎患者肝细胞染色体上存在整合型HBV，使HBV感染可能发生成为一种遗传性传染性疾病，并成为肝癌高发的重要危险因素。

To detect the integration of HBV on hepatacellular chromosome of the patients with chronic hepatitis B. using fluorescence in - situ hybridization 16 patients with chronic hepatitis B in our department were selected to set up the hepatacellular chromosome specimens. Chromosome in - situ hybridization was done with Dig - labeled HBV probe. Green fluorescent signals were seen in 5 patients specimens（31.25% ）. The integration sites were present in low frequency（ 1.00% - 1.50% ） and randomly distributied. Using fluorescence in - situ hybridization technique could detect HBV integration on the hepatacellular chromosome. HBV integrating into hepatacellular chromosome of the patients with chronic hepatitis B not only can lead to hereditary diseases, but also is a high risk factor for liver cancer.