Rosco纸片扩散法在生殖器念珠菌病体外药敏试验中的应用

Application of Rosco Disk Diffusion on Genital Candidiasis Susceptibility Test of Yeast-like Fungi

目的:研究Rosco纸片扩散法在生殖器念珠菌病药敏试验中的临床应用。方法:采用Rosco纸片扩散法和美国临床实验室标准化委员会(NCCLS)M27-A大量肉汤稀释法测定性病门诊分离的98株不同酵母样真菌菌株对氟康唑、伊曲康唑的药敏情况,并以3株酵母样菌株作质量控制。结果:两种方法所测酵母菌对氟康唑的总敏感率分别是87.8%和88.7%,完全符合率是95.9%。两种方法所测酵母菌对伊曲康唑的总敏感率分别是83.7%和84.7%,完全符合率是95.9%。结论:Rosco纸片扩散法具有操作简便,观察时间短、成本低廉等优点,在临床具有良好的实用性,能及时为临床医生在选择方案时提供参考。

Objective：The practical evaluation of rosco disk diffusion method on the genital candidiasis antifungal susceptibility test for yeast-like fungi. Methods：The fluconazole susceptibility test were detected by rosco disk diffusion method and NCCLS M27-abroth macrodiluion method, the total of 98 yeast-like fungi strains were isolated from genital candidiasis specimens. Three standard strains were used as the quality control. Results： The total susceptibility rates of yeasts to fluconazole by the two methods were 87.8% and 88.7% respectively. Coincidence rate of the two method was 95.9%. The total susceptibility rates of yeasts to itraconazole by the two method were 83.7% and 84.7% ,coincidence rate of the two method was 95.9%. Conclusion：Rosco disk diffusion method is simple, rapid and low cost. It is useful to screen for drug sensitivity test of patient with genital candidiasis.