摘要
去精清精子与外源DNA共孵育,使其携带外源DNA,再与成熟卵母细胞进行体外受精,生产转基因猪胚胎,为通过胚胎移植生产转基因猪奠定基础。结果:通过PCR从不同时期胚胎中检测出携带外源DNA的阳性胚,说明精子与外源DNA共孵育能使精子携带外源DNA,并通过体外受精技术使外源DNA进入早期胚胎。
Sperms which were removed of seminal fluid, were incubated with exogenous DNA, thus they can intake exogenous DNA and fertilize with matured oocytes in vitro. The purpose is to develop a way to produce early transgenic pig embryos at lower costs. It indicated that sperm incubated with exogenous DNA could intake the DNA and could be detected in embryos at different stages.
出处
《激光生物学报》
CAS
CSCD
2006年第2期175-178,共4页
Acta Laser Biology Sinica
基金
湖南省教育厅专项基金
湖南省财政厅专项基金
湖南省科技厅专项基金
湖南省发改委专项基金
关键词
精子载体
体外受精
转基因猪胚胎
sperm mediated gene transfer
in vitro fertilization
transgenic pig embryos
